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KMID : 0363320030240010134
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Journal of Korean Oriental Internal Medicine
2003 Volume.24 No. 1 p.134 ~ p.143
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Apoptosis and Anti-proliferaction by Saussurea lappa and Pharbitis nil in AGS Human Gastric Cancer Cell Line
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Ko Seong-Gyu
Oh Hee-Rah
Lee Sun-Dong
Hwang Gwi-Seo
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Abstract
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Objectives : We performed this study to understand the molecular basis of the antitumor effect of Saussurea lappa, Pharbitis nil, Plantago asiatica and Taraxacum mongolicum, which have been used for cancer treatment in Korean traditional medicine.
Design: We analyzed, the effect of these medicinal herbs on proliferation and apoptosis of tumor cells and its association with gene expression, We performed semi-quantitative reverse transcription-polymerase chain reaction(RT-PCR) analysis of cell cycle- and apoptosis-related genes using a gastric cancer cell line AGS.
Results : Cell counting assay and [3H]thymidine uptake analysis showed that Saussurea lappa and Pharbitis nil strongly inhibit cell proliferation of AGS in a dose-dependent manner. Interestingly, gene espression assay revealed that mRNA espression levels of c-Jun, c-Fos, c-Myc, and Cyclin D1 were markedly decreased by Saussurea lappa and Pharbitis nil. Furthermore, Saussurea lappa was identified to activate expression of the p53 tumor suppressor and its downstream effector p21{Wafl}, which leads to G_1 cell cycle arrest and apoptosis.
These observations suggest that the anticancer effect of Saussurea lappa and Pharbitis nil might be associated with their regulatory capability of tumor-related gene expression.
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KEYWORD
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Saussurea lappa, Pharbitis nil, antitumor effect, protooncogenes, p53
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